Otocinclus huaorani was described from streams in Ecuadorian Amazonia within the territory of the Huaorani indigenous people, for whom it is named. Ecuador's Amazonian lowlands contain a diverse loricariid fauna, and O. huaorani represents one of several Otocinclus species found in this region. It is a small, slender species with typical Otocinclus proportions and a pale brown-grey body with a defined lateral stripe.
The streams where O. huaorani occurs are characteristic of the upper Amazon piedmont — warm, soft, slightly acidic, and flowing through dense tropical rainforest. Submerged wood, leaf litter, and aquatic plants provide both shelter and grazing substrates. The species likely forages in groups in shallow, well-lit stream margins where algae and biofilm growth is most productive.
Aquarium keeping of O. huaorani is equivalent to that of other warm-water Amazonian otocinclus. The species is infrequently exported and primarily of interest to collectors building species-specific Otocinclus collections or crafting Ecuadorian biotope aquaria. Standard care applies: group housing, soft warm water, and supplemental vegetable feeding to ensure adequate nutrition.